About Me

Objective:

To strive towards perfection in the field of Agriculture Biotechnology and Computational Biology, highly confident about work, dedicated and deserving candidate wants to work with an organization that can explore my skills so that I can give my best to organization.  

Educational Qualifications:

S. No.	Examination passed	Year of Passing	Name of Uni./Board	Division	Marks (%)	Subject/ specialization
1.	M.Sc. (Agril. Biotech.)	2012	RAU*, Pusa	First	81.23	Agricultural Biotechnology
2.	B.Sc.	2009	Uni. of Rajasthan, Jaipur	First	72.22	Biotechnology Botany, Chemistry
3.	Intermediate	2005	RBSE, Ajmer	First	64.31	Sc. Biology
4.	High School	2003	RBSE, Ajmer	First	61.31	-

*Rajendra Agricultural University, Pusa, Bihar

Research Experience –

1.      One Month Training at Biotechnology Research & Development Laboratory of Indo- American Hybrid Seeds (India) Pvt. Ltd., Bangalore in the following fields :

·         Molecular Biology & r-DNA technology

·         Plant Tissue Culture

·         Protein related techniques

2.      M. Sc. Thesis

Sharma, N. K. (2012) ‘In Vitro Studies and Assessment of Genetic Relationships among Ocimum spp. by Using RAPD Markers’ M.Sc. Thesis Submitted to Rajendra Agricultural University, Pusa in partial fulfillment for the degree of M.Sc. (Agriculture Biotechnology).

3.      Currently Working with Plant-Virus Interaction Lab (PVIL), Mahyco Research Centre, Maharashtra Hybrid Seed Company Ltd. (MAHYCO), Jalna in 6 Month BCIL-DBT Project on “Serological and Molecular Identification of Wheat Infecting Viruses in India”

Fellowships –

·         D.B.T. (Department of Biotechnology), New Delhi provided fellowship during M.Sc. degree programme.

Publications:

1.      Vandana, Sharma, N.K. and Kumar, M. (2012). EST SSR markers analysis of in vitro regenerated plantlets of Festival and sweet Charlie cultivars of Strawberry. Intl. J. Agric. Env. Biotech. 5(4): 321-328.

2.      Sharma, N.K., Vandana and Kumar, M. (2012). Effect of 2,4-D, BAP,  KN, IAA and IBA on in vitro Regeneration of Ocimum canum Sims – An Important Hoary Basil Plant. (Accepted for publication in Biotechnology)

3.      Sharma, N.K., Vandana, Kumar, M. and Kumar, H. (2012). In-vitro Regeneration of Ocimum killimandscharicum L. - A Camphor Yielding Medicinal Plant. (Accepted for publication in RAU Journal of Research)

4.      Vandana, Sharma, N.K., Kumar, M. and Kumar, H. (2012). Direct In-vitro Shoot Multiplication of Garden Strawberry cv. Festival through Nodal Stem Culture. (Accepted for publication in RAU Journal of Research)

5.      Sharma, N.K., Vandana and Kumar, M. (2012). Micropropagation of Ocimum minimum L. - The Smallest Basil Plant. Biotechnology: An Indian Journal. 6(6): 185-189.

6.      Vandana, Sharma, N.K. and Kumar, M. (2012). Assessment of genetic fidelity of micropropagated clones of strawberry genotypes using EST-SSR markers. Biotechnology: An Indian Journal. 6(4, 5): 150-153.

7.      Sharma, N.K. (2012). Evaluation of Genetic Diversity among Six Important Indian Holy Basil Plants (Ocimum Species) using RAPD Markers. In: Proceedings of International Conference on Agricultural & Horticultural Sciences, Sept 14-15, 2012 Hyderabad, India. Journal of Agrotechnology. 1(2): 137.

8.      Sharma, N.K., Vandana and Kumar, M. (2012). EST-SSR Marker Analysis of Tissue Cultured Plantlets of Festival and Sweet Charlie Cultivars of Strawberry. In: Proceedings of International Conference on Agricultural & Horticultural Sciences, Sept 14-15, 2012 Hyderabad, India. Journal of Agrotechnology. 1(2): 123.

9.      Sharma, N.K., Vandana, Kumar, M. and Kumar, H. (2012). Assessment of genetic relationships among Ocimum species by using RAPD markers. In: National Seminar on “Recent Trends in Life Sciences”. Department of Botany, Patna University. pp. 52. Abstract.

10.  Sharma, N.K., Vandana, Kumar, M. and Kumar, H. (2012). Micropropagation of Six Ocimum species: An Important Medicinal Plants. In: National Seminar on “Recent Trends in Life Sciences”. Department of Botany, Patna University. pp : 53. Abstract.

11.  Vandana, Sharma, N.K., Kumar, M. and Kumar, H. (2012). In vitro Studies of Strawberry (Fragaria x ananassa Duch.) and Assessment of Clonal Fidelity using EST-SSR Markers. In: National Seminar on “Recent Trends in Life Sciences”. Department of Botany, Patna University. pp: 53. Abstract.

12.  Sharma, N.K. and Kumar, M. (2011). In vitro Studies of Six Ocimum spp. In: National Conference “Frontiers in Biological Sciences 4-5^th December”. VBS Purvanchal University, Jaunpur (U.P.). pp: 45-46. Abstract.

Presentations:

• Oral Presentation in National Conference “Frontiers in Biological Sciences 4-5^th Dec. 2011”at VBS Purvanchal University, Jaunpur (U.P.) of Research Paper Entitled “In vitro Studies of Six Ocimum spp.”
• Poster Presentation in “International Conference on Agricultural & Horticultural Sciences”, September 14-15, 2012, Hyderabad, India Organized by Omics Group, USA

Achievements:

• Qualified J.N.U. Combined Agriculture Biotechnology National Level Test, 2009 for M.Sc. and Occupied 88^th Rank in General Category.
• Created a biological Web Portal (NK BioSoft) for providing a collection of biological information’s viz. free available software’s, biological animations, biological databases, biological search engines, books etc. and visits from more than 55 countries have been registered.
• Reviewed and corrected various research papers of African Journal of Biotechnology (NAAS Rating - 7.5/10.0) as a regular review member of the Journal.

 Computer Skill:

·                   OS – Win XP, Vista & 7

Internet – Web designing (HTML, JavaScript), E-mail, Blogging (WordPress & Blogger), Use of Expasy tools, Genome Browsers (UCSC, NCBI etc.)

MS Office – Word, PowerPoint, Excel, Access, Publisher, Outlook.

Statistical Software’s –NTSYSpc Ver. 2.10, SPSS 16.0, MEGA 5.0, OpenStat, Phylip, POPGene,  SigmaStat etc.

Developed Websites:

1. NK BioSoft (www.nkbiosoft.co.in): Biological Web Portal.
2. Science Naukri (www.sciencenaukri.com): Science Academic Jobs, Conference’s, Seminar’s, Symposium’s Details.
3. Free BioTools (www.freebiotools.com): Free Biological Software’s Database (Direct Downloads and Online Data Analysis).

Biological Techniques Skill:

Molecular Markers: RFLP, RAPD, SSR, EST-SSR, ISSR and SNPs.

Plant Tissue Culture: Micropropagation, Embryo Culture, Anther Culture & Agrobacterium Mediated Plant Transformation.

Protein Related Work: SDS PAGE, Western Blotting & ELISA.

Molecular Biology: DNA & RNA Isolation, PCR, RT-PCR, DNA Sequencing.

Personal Information’s:

Father’s Name – Mr. Ganpat Lal Sharma

Mother’s Name – Mrs. Rameshwari Devi

D.O.B. & Place– 15-08-1988, Jaipur

Languages Knowledge – Hindi & English (Read, Speak & Write)

Nationality – Indian

I hereby declare that the particulars furnished above are true. I understand that my candidature will be cancelled if the information found to be false or incorrect.

Date:                                                                                                      (Nand Kishor Sharma)